The decision, which applies in Punjab territory, will end the act of actual checks for a flawless hymen and the obtrusive "two-finger test".
The Lahore High Court judge, Ayesha Malik said the tests were "embarrassing" and "had no criminological worth".
The decision followed two petitions recorded in Punjab territory by rights activists.
Campaigners have since quite a while ago requested a finish to virginity tests as a component of the clinical assessment in assault cases, saying they have no logical premise.
Monday's decision applies in Punjab however may fill in as a point of reference for petitions in other common high courts. A comparable appeal is at present forthcoming in the Sindh High Court.
Sameer Khosa, an attorney speaking to the candidates in the Lahore case, told the BBC the decision had "set up obviously that the virginity test has no criminological incentive regardless including sexual savagery".
Mr Khosa said he trusted the significant specialists would "reset their methods in the light of this decision and bid farewell to the virginity tests until the end of time".
What is a two-finger test?
The "two-finger test" is performed physically by embeddings a couple of fingers into a lady's vagina to test for its laxity and for the presence of a hymen - in principle to decide if the lady is explicitly dynamic and how much.
A few specialists guarantee the test can decide whether a lady has been entered unexpectedly, and the test has been utilized to dishonor casualties of assault who are decided to be explicitly capable.
The World Health Organization has completely exposed the test, saying it has no logical legitimacy and is a basic liberties infringement.
In her decision, Justice Malik said the test was "profoundly intrusive" and had "no logical or clinical prerequisite".
"It is a mortifying practice, which is utilized to project doubt on the person in question, instead of zeroing in on the blamed and the episode for sexual brutality," she said.
Sahar Bandial, one of the legal counselors who recorded the request in the Lahore case, said the tests were utilized to ruin ladies dependent on informal evaluations of their sexual history.
"There is a surmising that the lady is of simple ideals and prone to have assented to sexual movement," Ms Bandial said.
A worldwide issue
Ladies rights campaigners have since quite a while ago contended that the purported virginity test is important for a conventional male centric culture that movements fault onto ladies in case of rapes.
Generally couple of assaults are accounted for in Pakistan, as survivors can confront social disgrace. In cases that are accounted for, hardly any wrongdoers are indicted on the grounds that for powerless laws and convoluted methodology.
There was public shock in September after a lady in Lahore was assaulted before her kids when her vehicle stalled by the roadside. The case provoked fights the nation over and drove the president to support an intense new assault charge intended to accelerate preliminaries.
However, intrusive "virginity tests" proceed in Pakistan, and stay lawful in pieces of the nation not influenced by the decision in Lahore. The training has been in power in the South Asian locale since the pioneer period and has been recorded in any event 20 nations around the world, as indicated by the UN and WHO.
As of late, the two associations have lobbied for a finish to the training all around the world.
India banned the test in 2013, and its court request was cited by Justice Malik in her decision in Pakistan on Monday. Bangladesh prohibited the tests in 2018.
Afghanistan prohibited the training in 2018, however the nation's Independent Human Rights Commission said in September that ladies were all the while being compelled to go through the tests.
Reacting to Monday's decision, Pakistan's Federal Minister for Science and Technology, Chaudhry Fawad, invited what he called a "milestone administering".
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